Senior Experimental Officer
NMR Group
Department of Molecular Biology and Biotechnology
University of Sheffield
Firth Court
Western Bank
Sheffield S10 2TN
UK
Tel: (0114) 222 4323 (From outside UK: +44 114 222 4323)
Fax: (0114) 222 2800
email: C.J.Craven@shef.ac.uk
Photos from the NESBIC NMR relaxation workshop that I organised in April 2002 can be found here.
Feo - Transport of ferrous iron into bacteria
Cartron, M. L., Maddocks, S., Gillingham, P., Craven, C. J., Andrews, S. C.
BIOMETALS, 2006, 19 143-157
The denatured state under native conditions: A non-native-like collapsed state of N-PGK
Reed, M. A. C., Jelinska, C., Syson, K., Cliff, M. J., Splevins, A., Alizadeh, T., Hounslow, A. M., Staniforth, R. A., Clarke, A. R., Craven, C. J., Waltho, J. P.
J MOL BIOL, 2006, 357 365-372
Definable Equilibrium States in the Folding of Human Prion Protein
Hosszu, L. L. P., Wells, M. A., Jackson, G. S., Jones, S., Batchelor, M., Clarke, A. R., Craven, C. J., Waltho, J. P., Collinge, J.
BIOCHEMISY, 2005, 44 16649-16657
Obligate heterodimerization of the archaeal Alba2 protein with Alba1 provides a mechanism for control of DNA packaging
Jelinska, C., Conroy, M. J., Craven, C. J., Hounslow, A. M., Bullough, P. A., Waltho, J. P., Taylor, G. L., White, M. F.
STRUCTURE, 2005, 13, 963-971
Enhanced Ligand Affinity for Receptors in which Components of the Binding Site Are Independently Mobile
Trevitt, C. R., Craven, C. J., Milanesi, L., Syson, K., Mattinen, M., Perkins, J., Annila, A., Hunter, C. A., Waltho, J. P.
CHEMISTRY AND BIOLOGY, 2005, 12, 89-97
A Combinatorial Selective Labeling
Method for the Assignment of Backbone Amide NMR Resonances
Parker, M. J.,Aulton-Jones, M., Hounslow, A. M., Craven, C. J.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY; (Communication); 2004; 126(16);
5020-5021.
Phylogenetic
and evolutionary analysis of the PLUNC gene family
Bingle, C. D., LeClair, E. E., Havard, S., Bingle,
L., Gillingham, P., and Craven, C. J.
PROTEIN SCIENCE, 2004, 13 422-430
Cystatin
forms a tetramer through structural rearrangement of domain-swapped dimers
prior to amyloidogenesis
Sanders, A., Craven, C. J., Higgins, L. D., Giannini,
S., Conroy, M. J., Hounslow, A. M., Waltho, J. P., and Staniforth, R. A.
JOURNAL OF MOLECULAR BIOLOGY, 2004, 336 165-178.
Meet
the relatives: a family of BPI- and LBP-related proteins
Bingle, C. D., and Craven, C. J.
TRENDS IN IMMUNOLOGY, 2004, 25 53-55
Comparative
analysis of the PLUNC (palate, lung and nasal epithelium clone) protein
families
Bingle, C. D., and Craven, C. J.
BIOCHEMICAL SOCIETY TRANSACTIONS, 2003, 31 806-809
Effects
of domain dissection on the folding and stability of the 43 kDa protein
PGK probed by NMR
Reed, M. A. C., Hounslow, A. M., Sze, K. H.,
Barsukov, I. G., Hozzu, L., L., P., Clarke, A. R., Craven, C. J., and Waltho,
J. P.
JOURNAL OF MOLECULAR BIOLOGY, 2003, 330 1189-1201
PLUNC:
A novel family of candidate host defence proteins expressed in the upper
airways and nasopharynx
Bingle, C. D., and Craven, C. J.
HUMAN MOLECULAR GENETICS, 2002, 11 937-943
Three-dimensional
domain swapping in the folded and molten- globule states of cystatins,
an amyloid-forming structural superfamily
Staniforth R. A., Giannini S., Higgins L. D.,
Conroy M. J., Hounslow A. M., Jerala R., Craven C. J. and Waltho J. P.
EMBO JOURNAL, 2001, 20 4774-4781.
Structure
of TCTP reveals unexpected relationship with guanine nucleotide-free chaperones
Thaw P., Baxter N. J., Hounslow A. M., Price
C., Waltho J. P. and Craven C. J.
NATURE STRUCTURAL BIOLOGY, 2001, 8 701-704.
Wild-type
and Met-65 -> Leu variants of human cystatin A are functionally and structurally
identical
Craven C. J., Baxter N. J., Murray E. H., Hill
N. J., Martin J. R., Ylinenjarvi K., Bjork I., Waltho J. P. and Murray
I. A.
BIOCHEMISTRY, 2000, 39 15783-15790.
Probing
the nature of the blue-shifted intermediate of photoactive yellow protein
in solation by NMR: Hydrogen- deuterium exchange data and pH studies
Craven C. J., Derix N. M., Hendriks J., Boelens
R., Hellingwerf K. J. and Kaptein R.
BIOCHEMISTRY, 2000, 39 14392-14399.
Letter
to the Editor: Backbone NMR assignment of the 19 kDa translationally controlled
tumor-associated protein p23(fyp) from Schizosaccharomyces pombe
Baxter N. J., Thaw P., Higgins L. D., Sedelnikova
S. E., Bramley A. L., Price C., Waltho J. P. and Craven C. J.
JOURNAL OF BIOMOLECULAR NMR, 2000, 16 83-84.
Structural
mobility of the human prion protein probed by backbone hydrogen exchange
Hosszu L. L. P., Baxter N. J., Jackson G. S.,
Power A., Clarke A. R., Waltho J. P., Craven C. J. and Collinge J.
NATURE STRUCTURAL BIOLOGY, 1999, 6 740-743.
Identification
of amino acid residues critical for aggregation of human CC chemokines
macrophage inflammatory protein (MIP)-1 alpha, MIP-1 beta, and RANTES -
Characterization of active disaggregated chemokine variants
Czaplewski L. G., McKeating J., Craven C. J.,
Higgins L. D., Appay V., Brown A., Dudgeon T., Howard L. A., Meyers T.,
Owen J., Palan S. R., Tan P., Wilson G., Woods N. R., Heyworth C. M., Lord
B. I., Brotherton D., Christison R., Craig S., Cribbes S., Edwards R. M.,
Evans S. J., Gilbert R., Morgan P., Randle E., Schofield N., Varley P.
G., Fisher J., Waltho J. P. and Hunter M. G.
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 16077-16084.
Reversible conversion
of monomeric human prion protein between native and fibrilogenic conformations
Jackson GS, Hosszu LLP, Power A, Hill AF, Kenney J, Saibil H, Craven
CJ, Waltho JP, Clarke AR, Collinge J
SCIENCE, 1999, Vol.283, No.5409, pp.1935-1937
The role of
Gly-4 of human cystatin A (Stefin A) in the binding of target proteinases.
Characterization by kinetic and equilibrium methods of the interactions
of cystatin a Gly-4 mutants with papain, cathepsin B, and cathepsin L
Estrada S, Nycander M, Hill NJ, Craven CJ, Waltho JP, Bjork I
BIOCHEMISTRY, 1998, Vol.37, No.20, pp.7551-7560
Structure of a kinetic protein folding intermediate by equilibrium
amide exchange
Hosszu LLP, Craven CJ, Parker MJ, Lorch M, Spencer J, Clarke AR, Waltho
JP
NATURE STRUCTURAL BIOLOGY, 1997, Vol.4, No.10, pp.801-804
Is the structure of the N-domain of phosphoglycerate kinase affected
by isolation from the intact molecule?
Hosszu LLP, Craven CJ, Spencer J, Parker MJ, Clarke AR, Kelly M, Waltho
JP
BIOCHEMISTRY, 1997, Vol.36, No.2, pp.333-340
Domain behavior during the folding of a thermostable phosphoglycerate
kinase
Parker MJ, Spencer J, Jackson GS, Burston SG, Hosszu LLP, Craven CJ,
Waltho JP, Clarke AR
BIOCHEMISTRY, 1996, Vol.35, No.49, pp.15740-15752
Complexes formed between calmodulin and the antagonists J-8 and TFP
in solution
Craven CJ, Whitehead B, Jones SKA, Thulin E, Blackburn GM, Waltho JP
BIOCHEMISTRY, 1996, Vol.35, No.32, pp.10287-10299
The refined solution structure of human Stefin-A
Waltho JP, Martin JR, Craven CJ, Jerala R, Zerovnik E, Turk V
JOURNAL OF CELLULAR BIOCHEMISTRY, 1995, No.S21B, p.40
The binding of a hydrophobic drug to the C-terminal domain of calmodulin
Craven CJ, Jones SK, Evenas J, Drakenberg T, Finn B, Waltho JP
JOURNAL OF CELLULAR BIOCHEMISTRY, 1995, No.S21B, p.70
The action of time-domain convolution filters for solvent suppression
Craven CJ, Waltho JP
JOURNAL OF MAGNETIC RESONANCE SERIES B, 1995, Vol.106, No.1, pp.40-46
The 3-dimensional solution structure of human Stefin-A
Martin JR, Craven CJ, Jerala R, Kroonzitko L, Zerovnik E, Turk V, Waltho
JP
JOURNAL OF MOLECULAR BIOLOGY, 1995, Vol.246, No.2, pp.331-343
Molecular-dynamics study of reorientational motion in solid benzene
Craven CJ, Hatton PD, Pawley GS
MOLECULAR PHYSICS, 1993, Vol.79, No.6, pp.1227-1238
The structure and dynamics of solid benzene .1. A neutron powder
diffraction study of deuterated benzene from 4 K to the melting-point
Craven CJ, Hatton PD, Howard CJ, Pawley GS
JOURNAL OF CHEMICAL PHYSICS, 1993, Vol.98, No.10, pp.8236-8243
The structure and dynamics of solid benzene .2. Molecular- dynamics
studies
Craven CJ, Hatton PD, Pawley GS
JOURNAL OF CHEMICAL PHYSICS, 1993, Vol.98, No.10, pp.8244-8255
Melting of sulfur-hexafluoride clusters by molecular-dynamics simulation
Rousseau B, Boutin A, Fuchs AH, Craven CJ
MOLECULAR PHYSICS, 1992, Vol.76, No.5, pp.1079-1091
Molecular-dynamics of rigid polyatomic-molecules on transputer arrays
Craven CJ, Pawley GS
COMPUTER PHYSICS COMMUNICATIONS, 1991, Vol.62, No.2-3, pp.169- 178
Grain-boundary premelting in crystalline benzene as studied by proton
NMR
Craven CJ, Dosseh G, Rousseau B, Fuchs AH
JOURNAL DE PHYSIQUE, 1990, Vol.51, No.21, pp.2489-2499
1999 6 months working in the group of Rob Kaptein and Rolf Boelens at Utrecht University in The Netherlands (on leave from the University of Sheffield).
1995- Senior Experimental Officer, Department of Molecular Biology and Biotechnology, University of Sheffield
1993-1995 Research Associate, Department of Molecular Biology and Biotechnology, University of Sheffield
1991-1992 Lecturer, Department of Physics, University of Malawi, Zomba, Malawi (A VSO-like position)
1987-1991 PhD, Department of Physics, University of Edinburgh "A Molecular Dynamics Study of the Structure and Dynamics of Solid Benzene"
1984-1987 BA, Class I, Natural Sciences (Physics and Theoretical Physics), University of Cambridge
1984 Employed as a pre-University Student, Shell Research Ltd, Thornton Research Centre. NMR studies of lubricants.
1977-1984 Merchant Taylors' School, Crosby
1971-1977 St Jerome's School, Formby
I have created a number of pieces of software to augment our work locally. I have listed below some of these which find widespread use throughout our groups, and which may be of particular use to others. I am happy to distribute them on request. They are all highly UNIX scripting oriented.
jplot
genfit and boxplotjplot is a PostScript based plotting "package" that can be used to produce very complex graphs and other graphical representations of data such as sequence alignments. It is designed to be used with the output from a program in any scripting or programming language. It is based on the standard PostScript page description language, providing definitions of extra commands to take care of standard tasks such as setting up axes systems, labelling axes, drawing symbols and formatting text. The main component is just a big header for a postscript file, and a tiny shell script, and requires a standard PostScript viewer such as ghostview to view the output. As such its installation is trivial and can work on any UNIX (or Windows if you insisted) platform with a PostScript viewer. It also has an allied script jgraph (written in awk) which can be used in pipelines to get a quick look at data, and can then be built into scripts to build up more complex graphs. jplot was written as an alternative to parts of the Harwell FORTRAN GHOST library, which it rapidly superceded locally. It is unashamedly script oriented and has no GUI. There is no new scripting language to learn - you use your own favourite(s). With a little PostScript knowledge, it is infinitely extendable. There are no binary files involved, and the output is very readable PostScript. You would only want to use it if you are happy with scripting/programming, or "know someone who is" You might like to use it if, to get a quick look at data, you like the idea of a pipeline as simple as
nawk '{print $1,$2-$3}' datafile | jgraph | jplot2ps > temp.ps
or
my_application_that_generates_two_column_output | jgraph | jplot2ps > temp.ps
and if you dislike the alternatives of importing the data into a more GUI oriented program (and then reimporting it again and again .. and then forgetting what you did two years ago), or compiling a complex program with a graphics plotting library.The alignment figures in the TCTP and PLUNC papers were created using jplot, as were the graphs in the PYP paper.
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